Evaluation of biochar products at two inclusion levels on ruminal in vitro methane production and fermentation parameters in a Timothy hay-based diet

This study evaluated the effects of seven biochar products at two levels inclusion [2.25% or 4.50% diet dry matter (DM)] on DM disappearance (DMD), cumulative gas and methane (CH 4 ) production, ammonia-nitrogen, volatile fatty acid (VFA) production from Timothy grass hay over 48 h incubation. Biochar did not affect CH (P ≥ 0.17) nor DMD ruminal fermentation 0.12). In conclusion, biochar, irrespective level inclusion, exhibit potential to mitigate emission in a diet.